CXCL1 ELISA Kit (Rat) (OKCD05713)

Catalog No: OKCD05713

Size:96Wells

CXCL1 ELISA Kit (Rat) (OKCD05713) Standard Curve

Product Info

• Predicted Species Reactivity: Rat
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CXCL1 ELISA Kit (Rat) (OKCD05713) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXCL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXCL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CXCL1 captured in the well.
• ELISA Kit Range: 15.6-1000pg/mL
• ELISA Kit Reproducibility: Intra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CXCL1. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CXCL1 using 8 replicates on each of 3 plates.
  Intra-Assay CV <10%
  Inter-Assay CV <12%
• ELISA Kit Component:
  • CXCL1 Microplate: 96 Wells (12 x 8 Well strips)
  • CXCL1 Lyophilized Standard: 2
  • 100X CXCL1 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 6.7pg/mL
• Description:
  View product citations for CXCL1 ELISA Kit (Rat) (OKCD05713) on CiteAb

Target Info

• Gene Symbol: CXCL1
• Gene Full Name: C-X-C motif chemokine ligand 1
• Alias Symbols: Gro1, CINC-1
• NCBI Gene Id: 81503
• Protein Name: growth-regulated alpha protein
• Description of Target: acts as a neutrophil chemoattractant; may play a role in acute phase inflammatory response.
• Uniprot ID: P14095
• Protein Accession #: NP_110472.1
• Nucleotide Accession #: NM_030845.1