Catalog No: OKCD05713
Price: $439.00
Availability: Discontinued
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CXCL1 ELISA Kit (Rat) (OKCD05713) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CXCL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CXCL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CXCL1 captured in the well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level CXCL1. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level CXCL1 using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
CXCL1 Microplate96 Wells (12 x 8 Well strips)
CXCL1 Lyophilized Standard2
100X CXCL1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 6.7pg/mL
Gene SymbolCXCL1
Gene Full NameC-X-C motif chemokine ligand 1
Alias SymbolsGro1, CINC-1
NCBI Gene Id81503
Protein Namegrowth-regulated alpha protein
Description of Targetacts as a neutrophil chemoattractant; may play a role in acute phase inflammatory response.
Uniprot IDP14095
Protein Accession #NP_110472.1
Nucleotide Accession #NM_030845.1
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