CXCL1 Chemi-Luminescent ELISA Kit (Rat) (OKCD03821)

Catalog No: OKCD03821

Size:96WELLS

Rat CXCL1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	83-91%	92-99%	96-104%	78-98%
  EDTA Plasma (n=5)	97-105%	98-105%	97-105%	97-104%
  Heparin Plasma (n=5)	78-96%	95-102%	87-94%	87-97%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Chemokine (C-X-C Motif) Ligand 1 (CXCL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine (C-X-C Motif) Ligand 1 (CXCL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine (C-X-C Motif) Ligand 1 (CXCL1) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	80-101	90
  EDTA Plasma (n=5)	79-101	84
  Heparin Plasma (n=5)	78-89	83

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 1 (CXCL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine (C-X-C Motif) Ligand 1 (CXCL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Cxcl1 Microplate: 96 Wells (12 x 8 Well strips)
  • Cxcl1 Lyophilized Standard: 2
  • 100X Cxcl1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cxcl1
• Gene Full Name: C-X-C motif chemokine ligand 1
• Alias Symbols: chemokine (C-X-C motif) ligand 1 (melanoma growth stimulating activity, alpha);CINC-1;C-X-C motif chemokine 1;cytokine-induced neutrophil chemoattractant 1;gro;Gro1;growth-regulated alpha protein;platelet-derived growth factor-inducible protein KC.
• NCBI Gene Id: 81503
• Protein Name: Growth-regulated alpha protein
• Description of Target: Has chemotactic activity for neutrophils. Contributes to neutrophil activation during inflammation.
• Uniprot ID: P14095
• Protein Accession #: NP_110472
• Nucleotide Accession #: NM_030845