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Catalog No: OKCA00962
Size:96 Wells
Price: $754.00
SKU
OKCA00962
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat9287-95
1:210599-108
1:49085-93
1:89591-98
ELISA Kit PrincipleAviva Systems Biology CX3CR1 ELISA Kit (Rat) (OKCA00962) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for CX3CR1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CX3CR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CX3CR1 captured in well.
ELISA Kit Range23.5 pg/mL-1,500 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9287-94
EDTA Plasma (n=4)10799-111
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level CX3CR1 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level CX3CR1 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
CX3CR1 Microplate96 Wells (12 x 8 Well strips)
CX3CR1 Lyophilized Standard2
100X Biotinylated CX3CR1 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Lysates
Sensitivity5.8 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCX3CR1
Gene Full NameCX3C chemokine receptor 1
NCBI Gene Id100008874
Protein NameCX3C chemokine receptor 1
Description of TargetReceptor for the CX3C chemokine fractalkine (CX3CL1); binds to CX3CL1 and mediates both its adhesive and migratory functions.
Uniprot IDQ2KTE1
Protein Accession #NP_001075603.1
Nucleotide Accession #NM_001082134.1
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