Catalog No: OKCD07466
Size:96WELLS
Price: $750.00
SKU
OKCD07466
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for CX3CR1 ELISA Kit (Human) (OKCD07466) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X3-C-Motif Receptor 1 (CX3CR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-X3-C-Motif Receptor 1 (CX3CR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X3-C-Motif Receptor 1 (CX3CR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chemokine C-X3-C-Motif Receptor 1 (CX3CR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-X3-C-Motif Receptor 1 (CX3CR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-X3-C-Motif Receptor 1 (CX3CR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
Sensitivity | < 0.054ng/mL |
Gene Symbol | CX3CR1 |
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Gene Full Name | C-X3-C motif chemokine receptor 1 |
Alias Symbols | beta chemokine receptor-like 1;CCRL1;chemokine (C-C) receptor-like 1;chemokine (C-X3-C motif) receptor 1;chemokine (C-X3-C) receptor 1;CMKBRL1;CMK-BRL1;CMK-BRL-1;CMKDR1;CX3C chemokine receptor 1;C-X3-C CKR-1;fractalkine receptor;GPR13;G-protein coupled receptor 13;GPRV28;V28. |
NCBI Gene Id | 1524 |
Protein Name | CX3C chemokine receptor 1 |
Description of Target | Receptor for the CX3C chemokine fractalkine (CX3CL1); binds to CX3CL1 and mediates both its adhesive and migratory functions (PubMed:9390561, PubMed:23125415). Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro) (PubMed:9726990). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1 (PubMed:14607932). |
Uniprot ID | P49238 |
Protein Accession # | NP_001164642.1 |
Nucleotide Accession # | NM_001171171.1 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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