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Catalog No: OKCD07466
Size:96WELLS
Price: $750.00
SKU
OKCD07466
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Datasheets/ManualsPrintable datasheet for CX3CR1 ELISA Kit (Human) (OKCD07466)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X3-C-Motif Receptor 1 (CX3CR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-X3-C-Motif Receptor 1 (CX3CR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-X3-C-Motif Receptor 1 (CX3CR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chemokine C-X3-C-Motif Receptor 1 (CX3CR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-X3-C-Motif Receptor 1 (CX3CR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-X3-C-Motif Receptor 1 (CX3CR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CX3CR1 Microplate96 Wells (12 x 8 Well strips)
CX3CR1 Lyophilized Standard2
100X CX3CR1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.054ng/mL
Gene SymbolCX3CR1
Gene Full NameC-X3-C motif chemokine receptor 1
Alias Symbolsbeta chemokine receptor-like 1;CCRL1;chemokine (C-C) receptor-like 1;chemokine (C-X3-C motif) receptor 1;chemokine (C-X3-C) receptor 1;CMKBRL1;CMK-BRL1;CMK-BRL-1;CMKDR1;CX3C chemokine receptor 1;C-X3-C CKR-1;fractalkine receptor;GPR13;G-protein coupled receptor 13;GPRV28;V28.
NCBI Gene Id1524
Protein NameCX3C chemokine receptor 1
Description of TargetReceptor for the CX3C chemokine fractalkine (CX3CL1); binds to CX3CL1 and mediates both its adhesive and migratory functions (PubMed:9390561, PubMed:23125415). Acts as coreceptor with CD4 for HIV-1 virus envelope protein (in vitro) (PubMed:9726990). Isoform 2 and isoform 3 seem to be more potent HIV-1 coreceptors than isoform 1 (PubMed:14607932).
Uniprot IDP49238
Protein Accession #NP_001164642.1
Nucleotide Accession #NM_001171171.1
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