CX3CL1 Chemi-Luminescent ELISA Kit (Mouse) (OKCD03407)

Catalog No: OKCD03407

Size:96WELLS

Mouse CX3CL1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	88-97%	91-98%	91-105%	90-98%
  EDTA Plasma (n=5)	80-89%	79-93%	86-93%	81-93%
  Heparin Plasma (n=5)	94-102%	85-99%	85-95%	93-103%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) level in the sample or standard.
• ELISA Kit Range: 3.43-2,500pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	86-101	97
  EDTA Plasma (n=5)	79-93	86
  Heparin Plasma (n=5)	85-105	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Cx3cl1 Microplate: 96 Wells (12 x 8 Well strips)
  • Cx3cl1 Lyophilized Standard: 2
  • 100X Cx3cl1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 0.039 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Cx3cl1
• Gene Full Name: chemokine (C-X3-C motif) ligand 1
• Alias Symbols: AB030188;ABCD-3;AI848747;CX3C;CX3C membrane-anchored chemokine;C-X3-C motif chemokine 1;Cxc3;D8Bwg0439e;FK;fract;fractalkine;neuro;neurotactin;Scyd;Scyd1;small inducible cytokine subfamily D, 1;small-inducible cytokine D1.
• NCBI Gene Id: 20312
• Protein Name: Fractalkine
• Description of Target: Acts as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 and to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (By similarity). The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (PubMed:9177350, PubMed:10382755).
• Uniprot ID: O35188
• Protein Accession #: NP_001291321
• Nucleotide Accession #: NM_001304392