Catalog No: OKCD03406
Size:96WELLS
Price: $800.00
SKU
OKCD03406
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CX3CL1 Chemi-Luminescent ELISA Kit (Human) (OKCD03406)

Datasheets/ManualsPrintable datasheet for CX3CL1 Chemi-Luminescent ELISA Kit (Human) (OKCD03406)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)83-92%95-103%78-91%84-98%
EDTA Plasma (n=5)84-102%87-101%95-105%95-102%
Heparin Plasma (n=5)87-98%80-97%95-103%92-105%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Chemokine C-X3-C-Motif Ligand 1 (CX3CL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) level in the sample or standard.
ELISA Kit Range2.74-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)98-105102
EDTA Plasma (n=5)89-9692
Heparin Plasma (n=5)95-10398
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-X3-C-Motif Ligand 1 (CX3CL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CX3CL1 Microplate96 Wells (12 x 8 Well strips)
CX3CL1 Lyophilized Standard2
100X CX3CL1 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity31.2 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCX3CL1
Gene Full NameC-X3-C motif chemokine ligand 1
Alias SymbolsABCD-3;C3Xkine;chemokine (C-X3-C motif) ligand 1;CX3C membrane-anchored chemokine;C-X3-C motif chemokine 1;CXC3;CXC3C;fractalkine;neurotactin;NTN;NTT;SCYD1;small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin);small inducible cytokine subfamily D (Cys-X3-Cys), member-1;small-inducible cytokine D1.
NCBI Gene Id6376
Protein NameFractalkine
Description of TargetActs as a ligand for both CX3CR1 and integrins. Binds to CX3CR1 (PubMed:23125415, PubMed:9931005, PubMed:21829356). Binds to integrins ITGAV:ITGB3 and ITGA4:ITGB1. Can activate integrins in both a CX3CR1-dependent and CX3CR1-independent manner. In the presence of CX3CR1, activates integrins by binding to the classical ligand-binding site (site 1) in integrins. In the absence of CX3CR1, binds to a second site (site 2) in integrins which is distinct from site 1 and enhances the binding of other integrin ligands to site 1 (PubMed:23125415, PubMed:24789099). The soluble form is chemotactic for T-cells and monocytes and not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (PubMed:9024663, PubMed:9177350).
Uniprot IDP78423
Protein Accession #NP_001291321
Nucleotide Accession #NM_001304392
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