CX26 ELISA Kit (Human) (OKCD04463)

Catalog No: OKCD04463

Size:96WELLS

Human CX26 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	96-104%	98-105%	85-98%	80-104%
  EDTA Plasma (n=5)	93-101%	78-88%	81-98%	87-98%
  Heparin Plasma (n=5)	88-97%	95-105%	79-96%	90-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Connexin 26 (CX26). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Connexin 26 (CX26). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Connexin 26 (CX26), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Connexin 26 (CX26) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	86-98	90
  EDTA Plasma (n=5)	98-105	102
  Heparin Plasma (n=5)	99-105	102

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Connexin 26 (CX26) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Connexin 26 (CX26) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Sap Microplate: 96 Wells (12 x 8 Well strips)
  • Sap Lyophilized Standard: 2
  • 100X Sap HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: 0.66 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: GJB2
• Gene Full Name: gap junction protein beta 2
• Alias Symbols: BAPS;connexin 26;Connexin-26;CX26;DFNA3;DFNA3A;DFNB1;DFNB1A;gap junction beta 2 proteinc;gap junction beta-2 protein;gap junction protein, beta 2, 26kDa;HID;KID;mutant gap junction beta 2 protein;mutant gap junction protein beta 2;NSRD1;PPK.
• NCBI Gene Id: 2706
• Protein Name: Gap junction beta-2 protein
• Description of Target: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.
• Uniprot ID: P29033
• Protein Accession #: NP_003995.2
• Nucleotide Accession #: NM_004004.5