CUZD1 ELISA Kit (Human) (OKCD02476)

Catalog No: OKCD02476

Size:96WELLS

Human CUZD1 ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CUB And Zona Pellucida Like Domains Protein 1 (CUZD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CUB And Zona Pellucida Like Domains Protein 1 (CUZD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CUB And Zona Pellucida Like Domains Protein 1 (CUZD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CUB And Zona Pellucida Like Domains Protein 1 (CUZD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CUB And Zona Pellucida Like Domains Protein 1 (CUZD1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CUB And Zona Pellucida Like Domains Protein 1 (CUZD1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CUZD1 Microplate: 96 Wells (12 x 8 Well strips)
  • CUZD1 Lyophilized Standard: 2
  • 100X Biotinylated CUZD1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 6 mL
  • TMB Substrate: 1 x 9 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: 0.059 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of CUB And Zona Pellucida Like Domains Protein 1 (CUZD1). No significant cross-reactivity or interference between CUB And Zona Pellucida Like Domains Protein 1 (CUZD1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CUZD1
• Gene Full Name: CUB and zona pellucida like domains 1
• Alias Symbols: CUB and zona pellucida-like domain-containing protein 1;CUB and ZP domain-containing protein 1;ERG1;ERG-1;estrogen regulated gene 1;ITMAP1;transmembrane protein UO-44;UO-44.
• NCBI Gene Id: 50624
• Protein Name: CUB and zona pellucida-like domain-containing protein 1
• Description of Target: CUZD1 antiserum inhibits cell attachment and proliferation of ovarian cancer cells so may be involved in these processes. May also play a role in the uterus during late pregnancy and/or in trypsin activation in pancreatic acinar cells.
• Uniprot ID: Q86UP6
• Protein Accession #: NP_071317.2
• Nucleotide Accession #: NM_022034.5
• Protein Size (# AA): 607