CUL9 ELISA Kit (Human) (OKCD00971)

Catalog No: OKCD00971 (Formerly GWB-KBBHV5)

Size:96WELLS

Human CUL9 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cullin 9 (CUL9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cullin 9 (CUL9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cullin 9 (CUL9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cullin 9 (CUL9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cullin 9 (CUL9) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cullin 9 (CUL9) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CUL9 Microplate: 96 Wells (12 x 8 Well Strips)
  • CUL9 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated CUL9 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.116 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cullin 9 (CUL9). No significant cross-reactivity or interference between Cullin 9 (CUL9) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CUL9
• Gene Full Name: cullin 9
• Alias Symbols: CUL-9;cullin-9;H7AP1;p53-associated parkin-like cytoplasmic protein;PARC;parkin-like cytoplasmic p53 binding protein;UbcH7-associated protein 1.
• NCBI Gene Id: 23113
• Protein Name: Cullin-9
• Description of Target: Core component of a Cul9-RING ubiquitin-protein ligase complex, a complex that mediates ubiquitination and subsequent degradation of BIRC5 and is required to maintain microtubule dynamics and genome integrity. Acts downstream of the 3M complex, which inhibits CUL9 activity, leading to prevent ubiquitination of BIRC5 (PubMed:24793696). Cytoplasmic anchor protein in p53/TP53-associated protein complex. Regulates the subcellular localization of p53/TP53 and subsequent function (PubMed:12526791, PubMed:17332328).
• Uniprot ID: Q8IWT3
• Protein Accession #: NP_055904
• Nucleotide Accession #: NM_015089.3
• Protein Size (# AA): 2517