CUL7 Antibody (OAGA01048)

Catalog No: OAGA01048

Size:100 ul

Sample (30 ug of whole cell lysate)
A: Molt-4
7.5% SDS PAGE
OAGA01048 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded U87 xenograft, using Cullin 7(OAGA01048) antibody at 1:100 dilution.

Immunofluorescence analysis of methanol-fixed HeLa, using Cullin 7(OAGA01048) antibody at 1:200 dilution.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: ICC, IF, IHC-FFPE, WB
• Additional Information: Background: The protein encoded by this gene is a component of an E3 ubiquitin-protein ligase complex. The encoded protein interacts with TP53, CUL9, and FBXW8 proteins. Defects in this gene are a cause of 3M syndrome type 1 (3M1). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  Positive Controls: H1299, HeLa, HepG2, Molt-4, Raji
  Cellular Localization: Cytoplasm
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Recombinant fragment corresponding to a region within amino acids 1370 and 1698 of Cullin 7 (Uniprot ID#Q14999)
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 0.84 mg/ml
• Application Info: Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: CUL7
• Gene Full Name: Cullin-7
• Alias Symbols: 3M1, CUL-7, KIAA0076, dJ20C7.5
• NCBI Gene Id: 9820
• Protein Name: Cullin-7
• Uniprot ID: Q14999
• Protein Accession #: NP_001161842.1
• Molecular Weight: 191 kDa