CUEDC2 Antibody (OAPB00767)

Catalog No: OAPB00767

Size:100UG

Western blot analysis of CUEDC2 in HeLa cell lysate with CUEDC2 antibody at (A) 1 and (B) 2 ug/mL.

Immunocytochemistry of CUEDC2 in HeLa cells with CUEDC2 antibody at 5 ug/mL.

Product Info

• Predicted Species Reactivity: Bovine|Human|Mouse|Rat
• Product Format: Liquid. CUEDC2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: CUEDC2 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: CUEDC2 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human CUEDC2. The immunogen is located within the last 50 amino acids of CUEDC2.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-CUEDC2 antibody is Catalog # OEPB02889
• Application Info: CUEDC2 antibody can be used for detection of CUEDC2 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Other Applications Image 1 Data: Immunocytochemistry of CUEDC2 in HeLa cells with CUEDC2 antibody at 5 ug/mL.
• Reference: Shih SC, Prag G, Francis SA, et al. A ubiquitin-binding motif required for intramolecular monoubiquitylation, the CUE domain. EMBO J.2003; 22:1273-81.;Ponting CP. Proteins of the endoplasmic-reticulum-associated degradation pathway: domain detection and function prediction. Biochem. J.2000; 351:527-35.;Prag G, Misra S, Jones EA, et al. Mechanism of ubiquitin recognition by the CUE domain of Vps9p. Cell2003; 113:609-20.;Kang RS, Daniels CM, Francis SA, et al. Solution structure of a CUE-ubiquitin complex reveals a conserved mode of ubiquitin binding. Cell2003; 113:621-30.

Target Info

• Gene Symbol: CUEDC2
• Gene Full Name: CUE domain containing 2
• Alias Symbols: bA18I14.5;C10orf66;CUE domain-containing protein 2.
• NCBI Gene Id: 79004
• Protein Name: CUE domain-containing protein 2|CUE domain-containing protein 2 [Homo sapiens]
• Description of Target: Down-regulates ESR1 protein levels through the ubiquitination-proteasome pathway, regardless of the presence of 17 beta-estradiol. Also involved in 17 beta-estradiol-induced ESR1 degradation. Controls PGR protein levels through a similar mechanism.
• Uniprot ID: Q9H467
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/NP_076945
• Molecular Weight: Predicted: 32 kDa Observed: 34 kDa