CTXII ELISA Kit (Mouse) (OKEH03670)

Catalog No: OKEH03670

Size:96T

Mouse CTXII ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CTXII ELISA Kit (Mouse) (OKEH03670) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CTXII has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for CTXII is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CTXII captured in well.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 8.3% (n = 20)
  Mean Inter-assay CV%: <= 10.4% (n = 20)
• ELISA Kit Component:
  • CTXII Microplate: 96 Wells (12 x 8 Well strips)
  • CTXII Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated CTXII Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.085 ng/mL
• Specificity: Natural and recombinant Mouse C-telopeptide of Collagen alpha-1(II) chain
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CTXII
• Gene Full Name: C-telopeptide of Collagen alpha-1(II) chain
• Protein Name: C-telopeptide of Collagen alpha-1(II) chain
• Description of Target: In bone physiology, the C-terminal telopeptide (or more formally, carboxy-terminal collagen crosslinks, and known by the acronym CTX) is a serum biomarker used to measure the rate of bone turnover. It can be useful in assisting clinicians to determine a patient's nonsurgical treatment response as well as evaluate a patient's risk of developing complications during healing following surgical intervention. The test used to detect the CTX marker is called the Serum CrossLaps, and it is more specific to bone resorption than any other test currently available.