Catalog No: OKEH02711
Price: $600.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Competitive
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CTXII ELISA Kit (Human) (OKEH02711) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Human CTXII antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CTXII and incubated. The CTXII found in the sample or standards competes with the biotinylated CTXII for limited binding sites on the immobilized anti-Human CTXII antibody. Excess unbound biotinylated CTXII and sample or standard CTXII is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CTXII captured in the well and inversely proportional to the amount of CTXII which was contained in the sample or standard.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 7.5% (n = 20)
ELISA Kit Component
Anti-CTXII Microplate96 Wells (12 x 8 Well strips)
CTXII Lyophilized Standard2
Sample Diluent1 x 20 mL
100X CTXII-Biotin Complex1 x 60 uL
100X Avidin-HRP Conjugate1 x 120 uL
Biotin Complex Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity32 pg/mL
SpecificityNatural and recombinant Human C-telopeptide of Collagen alpha-1(II) chain

Extracellular matrix turnover and inflammation in chemically-induced TMJ arthritis mouse models. PLoS One. 14, e0223244 (2019). 31603905

Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolCTXII
Write Your Own Review
You're reviewing:CTXII ELISA Kit (Human) : 96 Wells (OKEH02711)
Your Rating
We found other products you might like!