CTSZ ELISA Kit (Human) (OKCD00361)

Catalog No: OKCD00361 (Formerly GWB-KBBYN5)

Size:96WELLS

Human CTSZ ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  serum(n=5)	84-103%	82-104%	94-105%	78-89%
  EDTA plasma(n=5)	94-102%	98-105%	82-101%	81-94%
  heparin plasma(n=5)	79-90%	88-101%	94-101%	80-93%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin Z (CTSZ). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin Z (CTSZ). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin Z (CTSZ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cathepsin Z (CTSZ) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	87-102	99
  EDTA plasma(n=5)	78-94	84
  heparin plasma(n=5)	93-105	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin Z (CTSZ) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin Z (CTSZ) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CTSZ Microplate: 96 Wells (12 x 8 Well Strips)
  • CTSZ Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated CTSZ Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.057 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cathepsin Z (CTSZ). No significant cross-reactivity or interference between Cathepsin Z (CTSZ) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CTSZ
• Gene Full Name: cathepsin Z
• Alias Symbols: carboxypeptidase LB;cathepsin B2;cathepsin IV;cathepsin P;cathepsin X;cathepsin Y;cathepsin Z;cathepsin Z1;CTSX;cysteine-type carboxypeptidase;lysosomal carboxypeptidase B;preprocathepsin P.
• NCBI Gene Id: 1522
• Protein Name: Cathepsin Z
• Description of Target: Exhibits carboxy-monopeptidase as well as carboxy-dipeptidase activity.
• Uniprot ID: Q9UBR2
• Protein Accession #: NP_001327
• Nucleotide Accession #: NM_001336.3
• Protein Size (# AA): 303