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Catalog No: OKCD00756 (Formerly GWB-KBBAC2)
Size:96WELLS
Price: $650.00
SKU
OKCD00756
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Datasheets/ManualsPrintable datasheet for CTSS ELISA Kit (Mouse) (OKCD00756)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)96-104%90-103%84-97%94-101%
EDTA plasma(n=5)78-99%81-98%82-94%80-101%
heparin plasma(n=5)90-101%81-90%87-105%84-97%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin S (CTSS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin S (CTSS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin S (CTSS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cathepsin S (CTSS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range31.2-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)90-9793
EDTA plasma(n=5)91-10196
heparin plasma(n=5)84-9188
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin S (CTSS) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin S (CTSS) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-Ctss Microplate96 Wells (12 x 8 Well Strips)
Ctss Lyophilized Standard2 x 2 ng
100X Biotinylated Ctss Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 12.2 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cathepsin S (CTSS). No significant cross-reactivity or interference between Cathepsin S (CTSS) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCtss
Gene Full Namecathepsin S
Alias SymbolsCat S;cathepsin S;Cats.
NCBI Gene Id13040
Protein NameCathepsin S
Description of TargetThiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L.
Uniprot IDO70370
Protein Accession #NP_067256
Nucleotide Accession #NM_021281.3
Protein Size (# AA)340
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