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Catalog No: OKEH06921
Size:96T
Price: $525.00
SKU
OKEH06921
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Ctsl ELISA Kit (Rat) (OKEH06921) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Ctsl has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Ctsl is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Ctsl captured in the well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into matrix = 100%
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <4.7%
Inter-Assay CV <8.2%
ELISA Kit Component
ComponentAmount
Ctsl Microplate96 Wells (12 x 8 Well strips)
Ctsl Lyophilized Standard2 x 20 ng
100X Ctsl HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.156 ng/mL
SpecificityNatural and recombinant Rat Cathepsin L1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCtsl
Gene Full Namecathepsin L
Alias SymbolsCathepsin L;cathepsin L1;CATHL;CatL;CP-2;Ctsl1;cyclic protein 2;major excreted protein;MEP;procathepsin L.
NCBI Gene Id25697
Protein NameP07154
Description of TargetCathepsin L1
Uniprot IDhttps://www.uniprot.org/uniprot/P07154
Protein Accession #NP_037288
Nucleotide Accession #NM_013156
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