Ctsg High Sensitivity ELISA Kit (Mouse) (OKCD01379)

Catalog No: OKCD01379

Size:96WELLS

Mouse Ctsg ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	98-105%	81-98%	84-91%	80-89%
  EDTA Plasma (n=5)	84-98%	82-104%	98-105%	88-95%
  Heparin Plasma (n=5)	99-105%	99-105%	89-97%	94-103%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Cathepsin G (CTSG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Cathepsin G (CTSG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Cathepsin G (CTSG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of High Sensitive Cathepsin G (CTSG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 3.12-200pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	78-90	81
  EDTA Plasma (n=5)	97-105	101
  Heparin Plasma (n=5)	80-103	99

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Cathepsin G (CTSG) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Cathepsin G (CTSG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-Ctsg Microplate: 96 Wells (12 x 8 Well Strips)
  • Ctsg Lyophilized Standard: 2
  • 100X Biotinylated Ctsg Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: 1.25 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cathepsin G (CTSG). No significant cross-reactivity or interference between Cathepsin G (CTSG) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Ctsg
• Gene Full Name: cathepsin G
• Alias Symbols: CatG;cathepsin G;vimentin-specific protease;VSP.
• NCBI Gene Id: 13035
• Protein Name: Cathepsin G
• Description of Target: This vimentin-specific protease may regulate the reorganization of vimentin filaments, occurring during cell differentiation, movement and mitosis.
• Uniprot ID: P28293
• Protein Accession #: NP_031826.1
• Nucleotide Accession #: NM_007800.2