CTSD ELISA Kit (Pig) (OKCA02486)

Catalog No: OKCA02486

Size:96 Wells

Pig CTSD ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Pig
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Dilution	Mean Recovery (%)	Range (%)
  Neat	89	85-94
  1:2	95	91-104
  1:4	94	86-97
  1:8	90	84-94

• ELISA Kit Principle: Aviva Systems Biology CTSD ELISA Kit (Pig) (OKCA02486) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CTSD has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CTSD is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard CTSD captured in the well.
• ELISA Kit Range: 15.6 - 1,000 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean Recovery (%)	Range (%)
  Serum (n=5)	91	84-95
  EDTA Plasma (n=4)	96	89-100

• ELISA Kit Reproducibility: Mean Intra-Assay: CV <12% (n=20)
  Mean Inter-Assay: CV <15% (n=24)
• ELISA Kit Component:
  • CTSD Microplate: 96 Wells (12 x 8 Well strips)
  • CTSD Lyophilized Standard: 2
  • 100X Biotinylated CTSD Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 10 mL
  • 25X Wash Buffer: 1 x 20 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Sensitivity: 3.9 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CTSD
• Alias Symbols: CLN10, CPSD, MGC2311, ceroid-lipofuscinosis, neuronal 10, lysosomal aspartyl peptidase, lysosomal aspartyl protease
• Protein Name: Cathepsin D
• Description of Target: Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation.
• Uniprot ID: P00795