Catalog No: OKCD07275
Size:96WELLS
Price: $750.00
SKU
OKCD07275
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Datasheets/ManualsPrintable datasheet for CTSD ELISA Kit (Human) (OKCD07275)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin D (CTSD). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin D (CTSD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin D (CTSD), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cathepsin D (CTSD) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.24-15ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin D (CTSD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin D (CTSD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CTSD Microplate96 Wells (12 x 8 Well strips)
CTSD Lyophilized Standard2
100X CTSD HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 0.055ng/mL
Gene SymbolCTSD
Gene Full Namecathepsin D
Alias Symbolscathepsin D;ceroid-lipofuscinosis, neuronal 10;CLN10;CPSD;epididymis secretory sperm binding protein Li 130P;HEL-S-130P;lysosomal aspartyl peptidase;lysosomal aspartyl protease.
NCBI Gene Id1509
Protein NameCathepsin D
Description of TargetAcid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation (PubMed:27333034). Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
Uniprot IDP07339
Protein Accession #NP_001900.1
Nucleotide Accession #NM_001909.4
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