CTSD Chemi-Luminescent ELISA Kit (Mouse) (OKCD03404)

Catalog No: OKCD03404

Size:96WELLS

Mouse CTSD ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	78-101%	80-97%	86-101%	79-99%
  EDTA Plasma (n=5)	78-94%	92-99%	94-101%	79-88%
  Heparin Plasma (n=5)	78-97%	83-97%	80-96%	89-101%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Cathepsin D (CTSD). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Cathepsin D (CTSD). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Cathepsin D (CTSD) level in the sample or standard.
• ELISA Kit Range: 2.74-2,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	82-90	87
  EDTA Plasma (n=5)	79-92	82
  Heparin Plasma (n=5)	90-104	96

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cathepsin D (CTSD) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cathepsin D (CTSD) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Ctsd Microplate: 96 Wells (12 x 8 Well strips)
  • Ctsd Lyophilized Standard: 2
  • 100X Ctsd Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 31.2 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Ctsd
• Gene Full Name: cathepsin D
• Alias Symbols: Cat;CatD;cathepsin D;CD.
• NCBI Gene Id: 13033
• Protein Name: Cathepsin D
• Description of Target: Acid protease active in intracellular protein breakdown. Plays a role in APP processing following cleavage and activation by ADAM30 which leads to APP degradation.
• Uniprot ID: P18242
• Protein Accession #: NP_034113
• Nucleotide Accession #: NM_009983