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Catalog No: OKEH00430
Price: $425.00
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CTSB ELISA Kit (Human) (OKEH00430) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Human CTSB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for Human CTSB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Human CTSB captured in well.
ELISA Kit Range0.312-20ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.3% (n = 20)
Mean Inter-assay CV%: < 7.6% (n = 20)
ELISA Kit Component
Anti-CTSB Microplate96 Wells (12 x 8 Well strips)
CTSB Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated CTSB1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.1 ng/mL
SpecificityNatural and recombinant Human Cathepsin B

Oral Fluid Biomarkers for Diagnosing Gingivitis in Human: A Cross-Sectional Study. J Clin Med. 9 (2020) 32503210

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCTSB
Gene Full Namecathepsin B
Alias Symbolsamyloid precursor protein secretase;APP secretase;APPS;cathepsin B;cathepsin B1;CPSB;cysteine protease;epididymis secretory sperm binding protein;RECEUP.
NCBI Gene Id1508
Protein NameP07858
Description of TargetCathepsin B
Uniprot ID
Protein Accession #NP_001304166.1
Nucleotide Accession #NM_001317237.1
Protein Size (# AA)110
Molecular Weight12 kDa
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