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Catalog No: OKEH01165
Size:96T
Price: $525.00
SKU
OKEH01165
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CTSA ELISA Kit (Human) : 96 Wells (OKEH01165)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CTSA ELISA Kit (Human) (OKEH01165) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CTSA has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CTSA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CTSA captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.7% (n = 20)
Mean Inter-assay CV%: < 8.1% (n = 20)
ELISA Kit Component
ComponentAmount
CTSA Microplate96 Wells (12 x 8 Well strips)
CTSA Lyophilized Standard2
100X Biotinylated CTSA Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity33 pg/mL
SpecificityNatural and recombinant Human Lysosomal protective protein
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCTSA
Gene Full Namecathepsin A
Alias Symbolsbeta-galactosidase 2;beta-galactosidase protective protein;carboxypeptidase C;Carboxypeptidase L;carboxypeptidase Y-like kininase;carboxypeptidase-L;Cathepsin A;deamidase;GLB2;GSL;lysosomal carboxypeptidase A;lysosomal protective protein;NGBE;PPCA;PPGB;protective protein cathepsin A;Protective protein for beta-galactosidase;urinary kininase.
NCBI Gene Id5476
Protein NameP10619
Description of TargetLysosomal protective protein
Uniprot IDhttps://www.uniprot.org/uniprot/P10619
Protein Accession #NP_000299.2
Nucleotide Accession #NM_000308.3
Protein Size (# AA)480
Molecular Weight54 kDa
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