CTNNBL1 ELISA Kit (Human) (OKWB00233)

Catalog No: OKWB00233

Size:96T

Human CTNNBL1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	95-103%	86-103%	85-102%	85-96%
  EDTA Plasma (n=5)	83-101%	88-101%	83-99%	85-98%
  Heparin Plasma (n=5)	80-93%	91-100%	83-98%	86-99%

• ELISA Kit Principle: Aviva Systems Biology CTNNBL1 ELISA Kit (Human) (OKWB00233) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CTNNBL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CTNNBL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CTNNBL1 captured in the well.
• ELISA Kit Range: 1.563-100ng/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	87-97	92
  EDTA Plasma (n=5)	88-104	94
  Heparin Plasma (n=5)	89-99	94

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • CTNNBL1 Microplate: 96 Wells (12 x 8 Well strips)
  • CTNNBL1 Lyophilized Standard: 2 x 100 ng
  • 100X CTNNBL1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.938 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CTNNBL1
• Gene Full Name: catenin beta like 1
• Alias Symbols: beta-catenin-like protein 1;C20orf33;dJ633O20.1;NAP;nuclear associated protein;Nuclear-associated protein;P14L;PP8304;testis development protein NYD-SP19.
• NCBI Gene Id: 56259
• Protein Name: Beta-catenin-like protein 1
• Description of Target: Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing. Participates in AID/AICDA-mediated Ig class switching recombination (CSR). May induce apoptosis.
• Uniprot ID: Q8WYA6
• Protein Accession #: NP_001268424
• Nucleotide Accession #: NM_001281495