Catalog No: OKCD02471
Size:96WELLS
Price: $650.00
SKU
OKCD02471
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Datasheets/ManualsPrintable datasheet for CTNNB1 ELISA Kit (Mouse) (OKCD02471)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)96-105%83-105%89-97%80-104%
EDTA Plasma (n=5)98-105%78-90%90-99%82-104%
Heparin Plasma (n=5)99-105%83-105%91-105%81-90%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta Catenin (Beta-catenin). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta Catenin (Beta-catenin). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta Catenin (Beta-catenin), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Beta Catenin (Beta-catenin) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)88-9793
EDTA Plasma (n=5)95-10399
Heparin Plasma(n=5)81-9788
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta Catenin (Beta-catenin) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta Catenin (Beta-catenin) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CTNNB1 Microplate96 Wells (12 x 8 Well strips)
CTNNB1 Lyophilized Standard2
100X Biotinylated CTNNB1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity5.9 pg/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Catenin Beta 1 (CTNNb1). No significant cross-reactivity or interference between Catenin Beta 1 (CTNNb1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolCtnnb1
Gene Full Namecatenin (cadherin associated protein), beta 1
Alias Symbolsbeta-catenin;Bfc;Cat;catenin beta-1;Catnb;Mesc.
NCBI Gene Id12387
Protein NameCatenin beta-1
Description of TargetKey downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (By similarity). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (PubMed:21325504).
Uniprot IDQ02248
Protein Accession #NP_001159374.1
Nucleotide Accession #NM_001165902.1
Protein Size (# AA)781
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