CTNNB1 Antibody - FITC Conjugated (OACA00119)

Catalog No: OACA00119

Size:100ug

All lanes : Catenin beta-1 antibody at at 2 ug/ml

Lane 1 :EC109 whole cell lysate
Lane 2 :293T whole cell lysate
Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size : 86 kDa
Observed band size: 86 kDa
Additional bands at : 27 kDa. We are unsure as to the identity of this extra band.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Conjugation: FITC
• Application: WB
• Reconstitution and Storage: -20°C or -80°C
• Immunogen: Recombinant Human Catenin beta-1 protein (2-781AA)
• Purification: Protein G Purified
• Purity: Caprylic Acid Ammonium Sulfate Precipitation
• Reference: [1] E-cadherin and APC compete for the interaction with beta-catenin and the cytoskeleton.'Huelsken J., Birchmeier W., Behrens J.J. Cell Biol. 127:2061-2069(1994)
• Storage Buffer: Preservative: 0.03% Proclin 300Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Target Info

• Gene Symbol: CTNNB1
• Gene Full Name: catenin beta 1
• Alias Symbols: armadillo;Beta-catenin;catenin (cadherin-associated protein), beta 1, 88kDa;catenin beta-1;CTNNB;EVR7;MRD19;NEDSDV.
• NCBI Gene Id: 1499
• Protein Name: Catenin beta-1
• Description of Target: Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex. Acts as a negative regulator of centrosome cohesion. Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization. Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2. Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22647378, PubMed:22699938, PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity).
• Uniprot ID: P35222