CTNNA1 Antibody (OAAF01170)

Catalog No: OAAF01170 (Formerly GWB-ASC563)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Catenin-alpha1 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Catenin-alpha1.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: Catenin-α1 antibody detects endogenous levels of total Catenin-α1 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:5000
• Reference: Yutaka Shimoyama. Cancer Res., Oct 1992; 52: 5770 - 5774.
  Griet Vanpoucke. Nucleic Acids Res., Aug 2004; 32: 4155 - 4165.
  Jolanda van Hengel. J. Cell Biol., Jun 1997; 137: 1103.

Target Info

• Gene Symbol: CTNNA1
• Gene Full Name: catenin alpha 1
• Alias Symbols: Alpha E-catenin;alpha-E-catenin;Cadherin-associated protein;CAP102;catenin (cadherin-associated protein), alpha 1, 102kDa;catenin alpha-1;epididymis secretory sperm binding protein;MDPT2;renal carcinoma antigen NY-REN-13.
• NCBI Gene Id: 1495
• Protein Name: Catenin alpha-1
• Description of Target: Associates with the cytoplasmic domain of a variety of cadherins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be of primary importance for cadherins cell-adhesion properties. Can associate with both E- and N-cadherins. Originally believed to be a stable component of E-cadherin/catenin adhesion complexes and to mediate the linkage of cadherins to the actin cytoskeleton at adherens junctions. In contrast, cortical actin was found to be much more dynamic than E-cadherin/catenin complexes and CTNNA1 was shown not to bind to F-actin when assembled in the complex suggesting a different linkage between actin and adherens junctions components. The homodimeric form may regulate actin filament assembly and inhibit actin branching by competing with the Arp2/3 complex for binding to actin filaments. May play a crucial role in cell differentiation.
• Uniprot ID: P35221
• Protein Accession #: NP_001894.2
• Molecular Weight: 100 kDa