CTLA4 Antibody - N-terminal region (OAAB00477)

Catalog No: OAAB00477 (Formerly GWB-HGIVJR)

Size:200ul

All lanes : Anti- CTLA4 Antibody (N-term) at 1:2000 dilution
Lane 1: WiDr whole cell lysate
Lane 2: Raji whole cell lysate
Lane 3: K562 whole cell lysate
Lane 4: Jurkat whole cell lysate
Lane 5: CCRF-CEM whole cell lysate.
Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 25 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes : Anti- CTLA4 Antibody (N-term) at 1:2000 dilution
Lane 1: Raji whole cell lysate
Lane 2: U266B1 whole cell lysate
Lane 3: K562 whole cell lysate
Lane 4: Jurkat whole cell lysate
Lane 5: CCRF-CEM whole cell lysate
Lane 6: Human spleen lysate
Lysates/proteins at 20 ug per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 25 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

CTLA4 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CTLA4 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Host: Rabbit
• Application: WB, IHC-P
  Function: Inhibitory receptor acting as a major negative regulator of T-cell responses. The affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28.
  Cellular Location: Cell membrane; Single-pass type I membrane protein. Note=Exists primarily an intracellular antigen whose surface expression is tightly regulated by restricted trafficking to the cell surface and rapid internalisation;
  Tissue Location: Widely expressed with highest levels in lymphoid tissues. Detected in activated T-cells where expression levels are 30- to 50-fold less than CD28, the stimulatory coreceptor, on the cell surface following activation.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This IDDM12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 50-78 amino acids of human IDDM12.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: 0.36 mg/ml
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:2000
  IHC-P: 1:50~100

Target Info

• Gene Symbol: CTLA4
• Alias Symbols: CD, GSE, GRD4, ALPS5, CD152, CTLA-4, IDDM12, CELIAC3
• NCBI Gene Id: 1493
• Protein Name: Cytotoxic T-lymphocyte protein 4
• Description of Target: CTLA4 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
• Uniprot ID: P16410
• Protein Accession #: NP_005205.2
• Molecular Weight: 25 kDa