Catalog No: OKCD02080 (Formerly GWB-KBBSG1)
Size:96 Wells
Price: $750.00
SKU
OKCD02080
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3.5 Hours
ELISA Kit PrincipleAviva Systems Biology Cthrc1 ELISA Kit (Mouse) (OKCD02080) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cthrc1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cthrc1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Cthrc1 captured in the well.
ELISA Kit Range0.156 - 10 ng/mL
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level Cthrc1 was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level Cthrc1 was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
ComponentAmount
Anti-Cthrc1 Microplate96 Wells (12 x 8 Well Strips)
Cthrc1 Lyophilized Standard2 x 10 ng
100X Biotinylated Cthrc1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.061 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Collagen Triple Helix Repeat Containing Protein 1 (CTHRC1). No significant cross-reactivity or interference between Collagen Triple Helix Repeat Containing Protein 1 (CTHRC1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCTHRC1
Gene Full NameCollagen triple helix repeat containing 1
Alias Symbols1110014B07Rik
NCBI Gene Id68588
Protein NameCollagen triple helix repeat-containing protein 1
Description of TargetMay act as a negative regulator of collagen matrix deposition.
Uniprot IDQ9D1D6
Protein Accession #NP_081054.1
Nucleotide Accession #NM_026778.2
Protein Size (# AA)245
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