Cthrc1 ELISA Kit (Mouse) (OKCD02080)

Catalog No: OKCD02080 (Formerly GWB-KBBSG1)

Size:96 Wells

Mouse Cthrc1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3.5 Hours
• ELISA Kit Principle: Aviva Systems Biology Cthrc1 ELISA Kit (Mouse) (OKCD02080) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cthrc1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cthrc1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Cthrc1 captured in the well.
• ELISA Kit Range: 0.156 - 10 ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level Cthrc1 was tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level Cthrc1 was tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10%
  Inter-Assay CV: <12%
• ELISA Kit Component:
  • Anti-Cthrc1 Microplate: 96 Wells (12 x 8 Well Strips)
  • Cthrc1 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated Cthrc1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.061 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Triple Helix Repeat Containing Protein 1 (CTHRC1). No significant cross-reactivity or interference between Collagen Triple Helix Repeat Containing Protein 1 (CTHRC1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CTHRC1
• Gene Full Name: Collagen triple helix repeat containing 1
• Alias Symbols: 1110014B07Rik
• NCBI Gene Id: 68588
• Protein Name: Collagen triple helix repeat-containing protein 1
• Description of Target: May act as a negative regulator of collagen matrix deposition.
• Uniprot ID: Q9D1D6
• Protein Accession #: NP_081054.1
• Nucleotide Accession #: NM_026778.2
• Protein Size (# AA): 245