CTGF ELISA Kit (Rat) : 96 Wells (OKEH00020)

Catalog No: OKEH00020

Size:96 Wells

Rat CTGF ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CTGF ELISA Kit (Rat) (OKEH00020) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CTGF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CTGF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CTGF captured in well.
• ELISA Kit Range: 15.6 - 1000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 4.9% (n = 20)
  Mean Inter-assay CV%: < 8.0% (n = 20)
• ELISA Kit Component:
  • CTGF Microplate: 96 Wells (12 x 8 Well strips)
  • CTGF Lyophilized Standard: 2
  • 100X Biotinylated CTGF Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 7.87 pg/mL
• Specificity: Natural and recombinant Rat Connective tissue growth factor
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CTGF
• Gene Full Name: connective tissue growth factor
• Alias Symbols: Ctgf, CTGRP
• NCBI Gene Id: 64032
• Protein Name: Connective tissue growth factor
• Description of Target: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin-and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis.
• Uniprot ID: Q9R1E9
• Protein Accession #: NP_071602
• Nucleotide Accession #: NM_022266
• Protein Size (# AA): 347
• Molecular Weight: 38 kDa