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Catalog No: OKEH00020
Size:96T
Price: $675.00
SKU
OKEH00020
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CTGF ELISA Kit (Rat) (OKEH00020) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CTGF has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CTGF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CTGF captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 4.9% (n = 20)
Mean Inter-assay CV%: < 8.0% (n = 20)
ELISA Kit Component
ComponentAmount
CTGF Microplate96 Wells (12 x 8 Well strips)
CTGF Lyophilized Standard2
100X Biotinylated CTGF Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity7.87 pg/mL
SpecificityNatural and recombinant Rat Connective tissue growth factor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolCcn2
Gene Full Namecellular communication network factor 2
Alias SymbolsCCN family member 2;connective tissue growth factor;connective tissue growth-related protein;Ctgf;CTGRP.
NCBI Gene Id64032
Protein NameQ9R1E9
Description of TargetConnective tissue growth factor
Uniprot IDhttps://www.uniprot.org/uniprot/Q9R1E9
Protein Accession #NP_071602
Nucleotide Accession #NM_022266
Protein Size (# AA)347
Molecular Weight38 kDa
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