CTGF Antibody (OABB02024)

Catalog No: OABB02024

Size:100UG

Anti-CTGF Picoband antibody, OABB02024, Western blotting
All lanes: Anti CTGF (OABB02024) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Thymus Tissue Lysate at 50ug
Lane 3: HELA Whole Cell Lysate at 40ug
Predicted bind size: 38KD
Observed bind size: 38KD

Anti-CTGF Picoband antibody, OABB02024, IHC(P)
IHC(P): Human Mammary Cancer Tissue

Product Info

• Tested Species Reactivity: Human, Rat
• Predicted Species Reactivity: Human|Rat
• Product Format: Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Clonality: Polyclonal
• Clone: Polyclonal
• Isotype: Rabbit IgG
• Host: Rabbit
• Application: Immunohistochemistry|Western blot
• Additional Information: Notes: Tested Species: In-house tested species with positive results.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
  Other applications have not been tested.
  Optimal dilutions should be determined by end users.
  Background: CTGF, also known as CCN2 or connective tissue growth factor, is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins (see also CCN intercellular signaling protein). CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. And the encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis.
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Immunogen: E.coli-derived human CTGF recombinant protein (Position: R58-A349). Human CTGF shares 95.5% and 95.9% amino acid (aa) sequence identity with mouse and rat CTGF, respectively.
• Purification: Affinity Purified
• Peptide Sequence: Synthetic peptide located within the following region: RVCAKQLGELCTERDPCDPHKGLFCHFGSPANRKIGVCTAKDGAPCIFGGTVYRSGESFQSSCKYQCTCLDGAVGCMPLCSMDVRLPSPDCPFPRRVKLPGKCCEEWVCDEPKDQTVVGPALAAYRLEDTFGPDPTMIRANCLVQTTEWSACSKTCGMGISTRVTNDNASCRLEKQSRLCMVRPCEADLEENIKKGKKCIRTPKISKPIKFELSGCTSMKTYRAKFCGVCTDGRCCTPHRTTTLPVEFKCPDGEVMKKNMMFIKTCACHYNCPGDNDIFESLYYRKMYGDMA
• Concentration: 500 ug/ml
• Specificity: No cross reactivity with other proteins.
• Application Info: Western blot: 0.1-0.5 ug/ml: Human, Rat
  Immunohistochemistry (Paraffin-embedded Section): 0.5-1 ug/ml: Human: By Heat
• Reference: 1. Chen CC, Lau LF (April 2009). "Functions and mechanisms of action of CCN matricellular proteins". Int. J. Biochem. Cell Biol. 41 (4): 771–83.
  2. Hall-Glenn F, Lyons KM (October 2011). "Roles for CCN2 in normal physiological processes". Cell. Mol. Life Sci. 68 (19): 3209–17.
  3. Jun JI, Lau LF (December 2011). "Taking aim at the extracellular matrix: CCN proteins as emerging therapeutic targets". Nat Rev Drug Discov 10 (12): 945–63.
  
• Storage Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Description: Rabbit IgG polyclonal antibody for Connective tissue growth factor(CTGF) detection. Tested with WB, IHC-P in Human;Rat.

Target Info

• Gene Symbol: CCN2
• Gene Full Name: cellular communication network factor 2
• Alias Symbols: CCN family member 2;connective tissue growth factor;CTGF;HCS24;hypertrophic chondrocyte-specific protein 24;IBP-8;IGF-binding protein 8;IGFBP8;IGFBP-8;insulin-like growth factor-binding protein 8;NOV2.
• NCBI Gene Id: 1490
• Protein Name: Connective tissue growth factor
• Description of Target: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis.
• Uniprot ID: P29279
• Molecular Weight: 38091 MW