CTCFL ELISA Kit (Human) (OKEH01659)

Catalog No: OKEH01659

Size:96T

Human CTCFL ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology CTCFL ELISA Kit (Human) (OKEH01659) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human CTCFL antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CTCFL and incubated. The CTCFL found in the sample or standards competes with the biotinylated CTCFL for limited binding sites on the immobilized anti-human CTCFL antibody. Excess unbound biotinylated CTCFL and sample or standard CTCFL is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CTCFL captured in the well and inversely proportional to the amount of CTCFL which was contained in the sample or standard.
  
• ELISA Kit Range: 0.312-20ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 6.3% (n = 20)
  Mean Inter-assay CV%: < 11.5% (n = 20)
• ELISA Kit Component:
  • Anti-CTCFL Microplate: 96 Wells (12 x 8 Well strips)
  • CTCFL Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X CTCFL-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.19 ng/mL
• Specificity: Natural and recombinant Human Transcriptional repressor CTCFL
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: CTCFL
• Gene Full Name: CCCTC-binding factor like
• Alias Symbols: BORIS;BORIS-like protein;brother of the regulator of imprinted sites;cancer/testis antigen 27;CCCTC-binding factor;CT27;CTCF paralog;CTCF-like protein;CTCF-T;dJ579F20.2;HMGB1L1;transcriptional repressor CTCFL;Zinc finger protein CTCF-T.
• NCBI Gene Id: 140690
• Protein Name: Q8NI51
• Description of Target: Transcriptional repressor CTCFL
• Uniprot ID: https://www.uniprot.org/uniprot/Q8NI51
• Protein Accession #: NP_001255969.1
• Nucleotide Accession #: NM_001269040.1
• Protein Size (# AA): 663
• Molecular Weight: 75 kDa