CT ELISA Kit (Chicken) (OKEH07400)

Catalog No: OKEH07400

Size:96T

Chicken CT ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Chicken, Gallus gallus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology CT ELISA Kit (OKEH07400) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-CT antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CT and incubated. The CT found in the sample or standards competes with the biotinylated CT for limited binding sites on the immobilized anti-CT antibody. Excess unbound biotinylated CT and sample or standard CT is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CT captured in the well and inversely proportional to the amount of CT which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.9% (n = 20)
  Mean Inter-assay CV%: <=8.0% (n = 20)
• ELISA Kit Component:
  • Anti-CT Microplate: 96 Wells (12 x 8 Well strips)
  • CT Lyophilized Standard: 2 x 100 ng
  • Sample Diluent: 1 x 20 mL
  • 100X CT-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Calcitonin (also known as thyrocalcitonin) is a 32-amino acid linear polypeptide hormone that is produced in humans primarily by the parafollicular cells (also known as C-cells) of the thyroid, and in many other animals in the ultimobranchial body. It acts to reduce blood calcium (Ca2+), opposing the effects of parathyroid hormone (PTH).
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.06ng/mL
• Specificity: Natural and recombinant Chicken Calcitonin
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: CT
• Protein Name: P07660
• Description of Target: Calcitonin
• Uniprot ID: https://www.uniprot.org/uniprot/P07660