CSTB Protein (OPPA01737)

Catalog No: OPPA01737 (Formerly GWB-BFEDC0)

Size:5UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: The protein solution contains 20mM Tris-HCl pH-8 & 50mM NaCl. Physical appearance: Sterile Filtered colorless solution.
• Host: E. Coli
  Product Introduction: Type 1 cystatins are also called stefins which function as intracellular thiol protease inhibitors. Cystatin-B protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. CSTB protein protects proteases leakage from lysosomes. Mutations in Stefin-B gene cause primary defects in patients with progressive myoclonic epilepsy (EPM1), a degenerative disease of the central nervous system. CSTB is overexpressed & elevated in the serum of HCC patients. Cystatin-B in vivo has a polymeric structure which is sensitive to the redox environment. Cystatin-B inhibits bone resorption by down-regulating intracellular cathepsin K activity despite increased osteoclast survival. Protein and mRNA levels of stefin B are significantly lower in atypical benign meningiomas. Stefins-A & Stefin-B which belong to the type-1 Cystatins, are up-regulated in lung tumours and thus able to counteract harmful tumour-associated proteolytic activity. Human stefin-A & Stefin-B form amyloid fibrils. Copper binding by stefin-B reduces amyloid fibril formation. A number of alternatively spliced CSTB isoforms were recognized in patients with progressive myoclonus epilepsy. Decreased CSTB activity in EPM1 pathogenesis is controled by cathepsins through increased activity of cathepsin-S & cathepsin-L.
  Product Description: CSTB Human Recombinant fused to His-Tag at N-Terminus produced in E.Coli is a single, non-glycosylated polypeptide chain containing 118 amino acids and having a molecular mass of 13 kDa.
• Reconstitution and Storage: Stefin-B although stable 4C for 4 weeks, should be stored desiccated below -18C. For long term storage it is recommended to add a carrier protein (0. 1% HSA or BSA). Please prevent freeze-thaw cycles.
• Purity: Greater than 95.0% as determined by SDS-PAGE.
• Peptide Sequence: MGSSHHHHHH SSGLVPRGSH MMCGAPSATQ PATAETQHIA DQVRSQLEEK ENKKFPVFKA VSFKSQVVAG TNYFIKVHVG DEDFVHLRVF QSLPHENKPL TLSNYQTNKA KHDELTYF.

Target Info

• Gene Symbol: CSTB
• Alias Symbols: PME, ULD, CST6, EPM1, STFB, CPI-B, EPM1A
• NCBI Gene Id: 1476
• Protein Name: Cystatin-B
• Description of Target: Recombinant Human Cystatin-B
• Uniprot ID: P04080
• Protein Accession #: NP_000091.1
• Protein Size (# AA): Recombinant