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Datasheets/Manuals | Printable datasheet for anti-CSTB (ARP45749_P050) antibody |
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Tested Species Reactivity | Human |
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Predicted Species Reactivity | Human, Mouse, Rat, Cow, Guinea Pig, Horse, Pig, Rabbit, Sheep |
Product Format | Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose. |
Clonality | Polyclonal |
Host | Rabbit |
Application | IHC, WB |
Additional Information | IHC Information: Transfected 293T cell lysate. Antibody concentration: 0.25 ug/ml. Gel concentration: 15%. |
Reconstitution and Storage | For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles. |
Immunogen | The immunogen is a synthetic peptide directed towards the N terminal region of human CSTB |
Purification | Affinity Purified |
Predicted Homology Based on Immunogen Sequence | Cow: 100%; Guinea Pig: 86%; Horse: 100%; Human: 100%; Mouse: 93%; Pig: 93%; Rabbit: 79%; Rat: 86%; Sheep: 100% |
Peptide Sequence | Synthetic peptide located within the following region: MMCGAPSATQPATAETQHIADQVRSQLEEKENKKFPVFKAVSFKSQVVAG |
Concentration | 0.5 mg/ml |
Blocking Peptide | For anti-CSTB (ARP45749_P050) antibody is Catalog # AAP45749 (Previous Catalog # AAPP26704) |
Reference | Kenig,M., (2006) Proteins 62 (4), 918-927 |
Gene Symbol | CSTB |
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Gene Full Name | Cystatin B (stefin B) |
Alias Symbols | PME, ULD, CST6, EPM1, STFB, CPI-B, EPM1A |
NCBI Gene Id | 1476 |
Protein Name | Cystatin-B |
Description of Target | CSTB is a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in CSTB gene are responsible for the primary defects in patients with progressive myoclonic epilepsy a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy.The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and kininogens. This gene encodes a stefin that functions as an intracellular thiol protease inhibitor. The protein is able to form a dimer stabilized by noncovalent forces, inhibiting papain and cathepsins l, h and b. The protein is thought to play a role in protecting against the proteases leaking from lysosomes. Evidence indicates that mutations in this gene are responsible for the primary defects in patients with progressive myoclonic epilepsy (EPM1). |
Uniprot ID | P04080 |
Protein Accession # | NP_000091 |
Nucleotide Accession # | NM_000100 |
Protein Size (# AA) | 98 |
Molecular Weight | 11kDa |
Protein Interactions | UBC; SARNP; DPP7; BAG3; CFTR; ECT2; SEC24A; NUDC; MAT2A; FLNB; DCUN1D1; CUL3; RAD21; SPRY2; CTSL; CTSD; CTSH; CTSB; CST3; VHL; |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "CSTB Antibody - N-terminal region (ARP45749_P050)"?
The tested species reactivity for this item is "Human". This antibody is predicted to have homology to "Human, Mouse, Rat, Cow, Guinea Pig, Horse, Pig, Rabbit, Sheep".
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How long will it take to receive "CSTB Antibody - N-terminal region (ARP45749_P050)"?
This item is available "Domestic: within 1-2 days delivery | International: 1-2 days".
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What buffer format is "CSTB Antibody - N-terminal region (ARP45749_P050)" provided in?
This item is provided in "Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "CSTB Antibody - N-terminal region (ARP45749_P050)"?
This target may also be called "PME, ULD, CST6, EPM1, STFB, CPI-B, EPM1A" in publications.
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What is the shipping cost for "CSTB Antibody - N-terminal region (ARP45749_P050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "CSTB Antibody - N-terminal region (ARP45749_P050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "CSTB Antibody - N-terminal region (ARP45749_P050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "11kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "CSTB Antibody - N-terminal region (ARP45749_P050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "CSTB"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "CSTB"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "CSTB"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "CSTB"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "CSTB"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "CSTB"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.