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Catalog No: OKCA01650
Size:96 Wells
Price: $754.00
SKU
OKCA01650
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityMouse
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean Recovery (%)Range (%)
Neat8984-93
1:210095-105
1:48783-90
1:8104100-107
ELISA Kit PrincipleAviva Systems Biology Cst7 ELISA Kit (Mouse) (OKCA01650) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cst7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cst7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Cst7 captured in the well.
ELISA Kit Range0.156 - 10 ng/mL
ELISA Kit Recovery
SampleMean Recovery (%)Range (%)
Serum (n=5)8683-89
EDTA Plasma (n=4)9591-99
ELISA Kit Component
ComponentAmount
Cst7 Microplate96 Wells (12 x 8 Well strips)
Cst7 Lyophilized Standard2 x 10 ng
100X Biotinylated Cst7 Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.039 ng/mL
Assay InfoAssay Methodology: Quantitative Sanadwich ELISA
Protocol Information
Gene SymbolCST7
Gene Full Namecystatin F (leukocystatin)
Alias SymbolsCM, Cmap
NCBI Gene Id13011
Protein NameCystatin-F
Description of TargetInhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.
Uniprot IDO89098
Protein Accession #NP_034107.2
Nucleotide Accession #NM_009977.3
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