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Catalog No: OKCD00565 (Formerly GWB-KBBCK6)
Size:96WELLS
Price: $675.00
SKU
OKCD00565
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Datasheets/ManualsPrintable datasheet for CST6 ELISA Kit (Human) (OKCD00565)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)90-102%96-105%79-89%81-94%
EDTA Plasma (n=5)90-98%81-95%92-99%78-93%
Heparin Plasma (n=5)94-105%80-88%98-105%81-104%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystatin 6 (CST6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cystatin 6 (CST6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cystatin 6 (CST6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cystatin 6 (CST6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)97-105101
EDTA plasma(n=5)82-8985
heparin plasma(n=5)80-10185
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cystatin 6 (CST6) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cystatin 6 (CST6) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CST6 Microplate96 Wells (12 x 8 Well Strips)
CST6 Lyophilized Standard2 x 10 ng
100X Biotinylated CST6 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, sweat, cell culture supernates and other biological fluids
Sensitivity< 0.067 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cystatin 6 (CST6). No significant cross-reactivity or interference between Cystatin 6 (CST6) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCST6
Gene Full Namecystatin E/M
Alias Symbolscystatin 6;cystatin M/E;Cystatin-6;cystatin-E;cystatin-M;cysteine proteinase inhibitor;ECTD15.
NCBI Gene Id1474
Protein NameCystatin-M
Description of TargetShows moderate inhibition of cathepsin B but is not active against cathepsin C.
Uniprot IDQ15828
Protein Accession #NP_001314
Nucleotide Accession #NM_001323.3
Protein Size (# AA)149
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