CST4 ELISA Kit (Human) (OKEH07972)

Catalog No: OKEH07972

Size:96T

Human CST4 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	108-116% (n = 20)	115-125% (n = 20)	90-100% (n = 20)	99-108% (n = 20)
  EDTA plasma(n=5)	86-97% (n = 20)	108-119% (n = 20)	94-105% (n = 20)	107-118% (n = 20)
  heparin plasma(n=5)	82-90% (n = 20)	92-102% (n = 20)	103-113% (n = 20)	108-118% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology CST4 ELISA Kit (Human) (OKEH07972) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CST4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CST4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CST4 captured in well.
• ELISA Kit Range: 0.78-50ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=4.2% (n = 20)
  Mean Inter-assay CV%: <=5.7% (n = 20)
• ELISA Kit Component:
  • CST4 Microplate: 96 Wells (12 x 8 Well strips)
  • CST4 Lyophilized Standard: 2
  • 100X Biotinylated CST4 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively.
  Subcellular Location: Secreted
  Unigene: Hs.654549
  SMR: P01036
  String: 9606.ENSP00000217423
  KEGG: hsa:1472
  MIM: 123857
  Pfam: PF00031
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.33ng/mL
• Specificity: Natural and recombinant Human Cystatin-S
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CST4
• Gene Full Name: cystatin S
• Alias Symbols: cystatin 4;Cystatin-4;cystatin-S;cystatin-SA-III;salivary acidic protein 1.
• NCBI Gene Id: 1472
• Protein Name: P01036
• Description of Target: Cystatin-S
• Uniprot ID: https://www.uniprot.org/uniprot/P01036
• Protein Accession #: NP_001890.1
• Nucleotide Accession #: NM_001899.2