CST4 ELISA Kit (Human) (OKCD00564)

Catalog No: OKCD00564 (Formerly GWB-KBBSV5)

Size:96WELLS

Human CST4 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	86-98%	97-105%	85-92%	87-101%
  EDTA Plasma (n=5)	89-99%	80-104%	95-105%	81-102%
  Heparin Plasma (n=5)	97-105%	83-97%	81-89%	78-98%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cystatin 4 (CST4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cystatin 4 (CST4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cystatin 4 (CST4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cystatin 4 (CST4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  serum(n=5)	82-95	89
  EDTA plasma(n=5)	84-92	89
  heparin plasma(n=5)	78-99	87

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cystatin 4 (CST4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cystatin 4 (CST4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-CST4 Microplate: 96 Wells (12 x 8 Well Strips)
  • CST4 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated CST4 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Sensitivity: < 0.063 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cystatin 4 (CST4). No significant cross-reactivity or interference between Cystatin 4 (CST4) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: CST4
• Gene Full Name: cystatin S
• Alias Symbols: cystatin 4;Cystatin-4;cystatin-S;cystatin-SA-III;salivary acidic protein 1.
• NCBI Gene Id: 1472
• Protein Name: Cystatin-S
• Description of Target: This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively.
• Uniprot ID: P01036
• Protein Accession #: NP_001890
• Nucleotide Accession #: NM_001899.2
• Protein Size (# AA): 141