CST3 ELISA Kit (Pig) (OKDD00620)

Catalog No: OKDD00620 (Formerly GWB-KBBFC3)

Size:96 Wells

Pig CST3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Porcine|Sus scrofa
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology CST3 ELISA Kit (Pig) (OKDD00620) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody for CST3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CST3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CST3 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision: 3 samples with low, middle and high level analyte were measured with 20 replicates on one plate, respectively.

  Inter-assay Precision: 3 samples with low, middle and high level analyte were measured across 3 different plates, with 8 replicates per plate.

  Mean Intra-Assay: CV<10%

  Mean Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-CST3 Microplate: 96 Wells (12 x 8 Well Strips)
  • CST3 Lyophilized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X CST3 Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma, urine and other biological fluids.
• Sensitivity: < 0.056 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of CST3. No significant cross-reactivity or interference between CST3 and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CST3
• Gene Full Name: cystatin C
• Alias Symbols: cystatin-C.
• NCBI Gene Id: 733672
• Protein Name: Cystatin
• Uniprot ID: Q0Z8R0
• Protein Accession #: NP_001038067.1
• Nucleotide Accession #: NM_001044602.1
• Protein Size (# AA): 146
• Molecular Weight: 16 kDa