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Catalog No: OKEH08342
Size:96T
Price: $725.00
SKU
OKEH08342
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology CSRP3 ELISA Kit (Human) (OKEH08342) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CSRP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CSRP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CSRP3 captured in well.
ELISA Kit Range0.156-10ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 105% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=7.8% (n = 20)
Mean Inter-assay CV%: <=12.2% (n = 20)
ELISA Kit Component
ComponentAmount
CSRP3 Microplate96 Wells (12 x 8 Well strips)
CSRP3 Lyophilized Standard2
100X Biotinylated CSRP3 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Isoform 2: May play a role in early sarcomere organization. Overexpression in myotubes negatively regulates myotube differentiation. By association with isoform 1 and thus changing the CSRP3 isoform 1:CFL2 stoichiometry is proposed to down-regulate CFL2-mediated F-actin depolymerization.
::Subcellular Location: Isoform 2 Cytoplasm Myofibril Sarcomere Z line
::Unigene: Hs.83577
SMR: P50461
String: 9606.ENSP00000265968
::KEGG: hsa:8048
MIM: 600824
Pfam: PF00412
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.078ng/mL
SpecificityNatural and recombinant Human Cysteine and glycine-rich protein 3
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCSRP3
Gene Full Namecysteine and glycine rich protein 3
Alias Symbolscardiac LIM domain protein;Cardiac LIM protein;CLP;CMD1M;CMH12;CRP3;cysteine and glycine-rich protein 3;cysteine and glycine-rich protein 3 (cardiac LIM protein);Cysteine-rich protein 3;LIM domain only 4;LIM domain protein, cardiac;LMO4;MLP;Muscle LIM protein.
NCBI Gene Id8048
Protein NameP50461
Description of TargetCysteine and glycine-rich protein 3
Uniprot IDhttps://www.uniprot.org/uniprot/P50461
Protein Accession #NP_003467.1
Nucleotide Accession #NM_003476.4
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