Catalog No: OKEH08342
Size:96T
Price: $725.00
SKU
OKEH08342
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
---|
Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology CSRP3 ELISA Kit (Human) (OKEH08342) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CSRP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CSRP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CSRP3 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=7.8% (n = 20) Mean Inter-assay CV%: <=12.2% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
| ||||||||||||||||||||||
Additional Information | Function: Isoform 2: May play a role in early sarcomere organization. Overexpression in myotubes negatively regulates myotube differentiation. By association with isoform 1 and thus changing the CSRP3 isoform 1:CFL2 stoichiometry is proposed to down-regulate CFL2-mediated F-actin depolymerization. | ||||||||||||||||||||||
:: | Subcellular Location: Isoform 2 Cytoplasm Myofibril Sarcomere Z line | ||||||||||||||||||||||
:: | Unigene: Hs.83577 SMR: P50461 String: 9606.ENSP00000265968 | ||||||||||||||||||||||
:: | KEGG: hsa:8048 MIM: 600824 Pfam: PF00412 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 0.078ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Cysteine and glycine-rich protein 3 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | CSRP3 |
---|---|
Gene Full Name | cysteine and glycine rich protein 3 |
Alias Symbols | cardiac LIM domain protein;Cardiac LIM protein;CLP;CMD1M;CMH12;CRP3;cysteine and glycine-rich protein 3;cysteine and glycine-rich protein 3 (cardiac LIM protein);Cysteine-rich protein 3;LIM domain only 4;LIM domain protein, cardiac;LMO4;MLP;Muscle LIM protein. |
NCBI Gene Id | 8048 |
Protein Name | P50461 |
Description of Target | Cysteine and glycine-rich protein 3 |
Uniprot ID | https://www.uniprot.org/uniprot/P50461 |
Protein Accession # | NP_003467.1 |
Nucleotide Accession # | NM_003476.4 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
Write Your Own Review