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Catalog No: OKCD07170
Size:96WELLS
Price: $750.00
SKU
OKCD07170
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Datasheets/ManualsPrintable datasheet for CSPG4 ELISA Kit (Human) (OKCD07170)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chondroitin Sulfate Proteoglycan 4 (CSPG4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chondroitin Sulfate Proteoglycan 4 (CSPG4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chondroitin Sulfate Proteoglycan 4 (CSPG4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Chondroitin Sulfate Proteoglycan 4 (CSPG4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chondroitin Sulfate Proteoglycan 4 (CSPG4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chondroitin Sulfate Proteoglycan 4 (CSPG4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CSPG4 Microplate96 Wells (12 x 8 Well strips)
CSPG4 Lyophilized Standard2
100X CSPG4 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.056ng/mL
Gene SymbolCSPG4
Gene Full Namechondroitin sulfate proteoglycan 4
Alias Symbolschondroitin sulfate proteoglycan 4;chondroitin sulfate proteoglycan 4 (melanoma-associated);chondroitin sulfate proteoglycan NG2;CSPG4A;HMW-MAA;MCSP;MCSPG;melanoma chondroitin sulfate proteoglycan;melanoma-associated chondroitin sulfate proteoglycan;MEL-CSPG;MSK16;NG2.
NCBI Gene Id1464
Protein NameChondroitin sulfate proteoglycan 4
Description of TargetProteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
Uniprot IDQ6UVK1
Protein Accession #NP_001888.2
Nucleotide Accession #NM_001897.4
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