Catalog No: OKEH08157
Size:96T
Price: $800.00
SKU
OKEH08157
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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CSNK2A2 ELISA Kit (Bovine) (OKEH08157)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityBos taurus, Bovine
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology CSNK2A2 ELISA Kit (Bovine) (OKEH08157) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CSNK2A2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CSNK2A2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CSNK2A2 captured in well.
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 107% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=5.4% (n = 20)
Mean Inter-assay CV%: <=12.1% (n = 20)
ELISA Kit Component
ComponentAmount
CSNK2A2 Microplate96 Wells (12 x 8 Well strips)
CSNK2A2 Lyophilized Standard2
100X Biotinylated CSNK2A2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation. Can also negatively regulate apoptosis. Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3. Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8. Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV. Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB. Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function. Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1. Acts as an ectokinase that phosphorylates several extracellular proteins.
::Unigene: Bt.1408
String: 9913.ENSBTAP00000018322
::KEGG: bta:282420
Pfam: PF00069
Reconstitution and StorageStore as indicated in product manual.
SpecificityNatural and recombinant Bovine Casein kinase II subunit alpha'
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCSNK2A2
Gene Full Namecasein kinase 2 alpha 2
Alias Symbolscasein kinase 2, alpha prime polypeptide;casein kinase II subunit alpha';CK II alpha'.
NCBI Gene Id282420
Protein NameP20427
Description of TargetCasein kinase II subunit alpha'
Uniprot IDhttps://www.uniprot.org/uniprot/P20427
Protein Accession #NP_777061.1
Nucleotide Accession #NM_174636.2
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