Catalog No: OKCD01122 (Formerly GWB-KBBJK9)
Size:96WELLS
Price: $675.00
SKU
OKCD01122
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Datasheets/ManualsPrintable datasheet for CSN3 ELISA Kit (Human) (OKCD01122)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Casein Kappa (CSN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Casein Kappa (CSN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Casein Kappa (CSN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Casein Kappa (CSN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range78-5,000pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Casein Kappa (CSN3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Casein Kappa (CSN3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-CSN3 Microplate96 Wells (12 x 8 Well Strips)
CSN3 Lyophilized Standard2 x 200 ng
100X Biotinylated CSN3 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeBreast milk and other biological fluids
Sensitivity< 1.31 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Casein Kappa (CSN3). No significant cross-reactivity or interference between Casein Kappa (CSN3) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCSN3
Gene Full Namecasein kappa
Alias SymbolsCNS10;CSN10;CSNK;kappa-casein;KCA.
NCBI Gene Id1448
Protein NameKappa-casein
Description of TargetKappa-casein stabilizes micelle formation, preventing casein precipitation in milk.
Uniprot IDP07498
Protein Accession #NP_776719
Nucleotide Accession #NM_174294.2
Protein Size (# AA)182
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