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Catalog No: OKCD09187
Size:96WELLS
Price: $800.00
SKU
OKCD09187
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Datasheets/ManualsPrintable datasheet for CSN2 ELISA Kit (Human) (OKCD09187)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Casein Beta (CSN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Casein Beta (CSN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Casein Beta (CSN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Casein Beta (CSN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range7.8-500ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Casein Beta (CSN2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Casein Beta (CSN2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CSN2 Microplate96 Wells (12 x 8 Well strips)
CSN2 Lyophilized Standard2
100X CSN2 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeBreast milk and other biological fluids
Sensitivity< 0.069ng/mL
Gene SymbolCSN2
Gene Full Namecasein beta
Alias Symbolsbeta-casein;CASB;PDC213.
NCBI Gene Id1447
Protein NameBeta-casein
Description of TargetImportant role in determination of the surface properties of the casein micelles.
Uniprot IDP05814
Protein Accession #NP_001289699.1
Nucleotide Accession #NM_001302770.1
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