CSK Antibody (OAEE00364)

Catalog No: OAEE00364

Size:100UL

Immunofluorescence staining of Csk in human HeLa cell line using anti-Csk (CSK-04; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: CSK-04
• Isotype: IgG1
• Host: Mouse
• Application: Immunocytochemistry|Immunoprecipitation|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Bacterially expressed recombinant fragment of human Csk corresponding to aa 330-450.
• Purification: Purified
• Specificity: The antibody CSK-04 reacts with Csk (C-terminal Src kinase), a 50 kDa non-receptor protein tyrosine kinase expressed in cytosol.
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Liang F, Luo Y, Dong Y, Walls CD, Liang J, Jiang HY, Sanford JR, Wek RC, Zhang ZY: Translational Control of C-terminal Src Kinase (Csk) Expression by PRL3Phosphatase. J Biol Chem. 2008 Apr 18;283(16):10339-46.
  Ingley E: Src family kinases: regulation of their activities, levels and identification of new pathways. Biochim Biophys Acta. 2008 Jan;1784(1):56-65.
  Takayama Y, Tanaka S, Nagai K, Okada M: Adenovirus-mediated overexpression of C-terminal Src kinase (Csk) in type Iastrocytes interferes with cell spreading and attachment to fibronectin. Correlation with tyrosine phosphorylations of paxillin and FAK. J Biol Chem. 1999 Jan 22;274(4):2291-7.
  Bergman M, Joukov V, Virtanen I, Alitalo K: Overexpressed Csk tyrosine kinase is localized in focal adhesions, causes reorganization of alpha v beta 5 integrin, and interferes with HeLa cell spreading. Mol Cell Biol. 1995 Feb;15(2):711-22.
  Chow LM, Fournel M, Davidson D, Veillette A: Negative regulation of T-cell receptor signalling by tyrosine protein kinase p50csk. Nature. 1993 Sep 9;365(6442):156-60.
  Sabe H, Okada M, Nakagawa H, Hanafusa H: Activation of c-Src in cells bearing v-Crk and its suppression by Csk. Mol Cell Biol. 1992 Oct;12(10):4706-13.

Target Info

• Gene Symbol: CSK
• Gene Full Name: C-terminal Src kinase
• Alias Symbols: CSK, non-receptor tyrosine kinase;C-Src kinase;c-src tyrosine kinase;protein-tyrosine kinase CYL;tyrosine-protein kinase CSK.
• NCBI Gene Id: 1445
• Protein Name: Tyrosine-protein kinase CSK
• Description of Target: Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.
• Uniprot ID: P41240
• Protein Accession #: NP_001120662.1; NP_004374.1; XP_005254222.1
• Nucleotide Accession #: NM_001127190.1; NM_004383.2; XM_005254165.3