CSK Antibody (OAEB02339)

Catalog No: OAEB02339 (Formerly GWB-D4DEB0)

Size:100UG

EB5118 (0.5ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02339 (1ug/ml) staining of Mouse Spleen lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

OAEB02339 (4ug/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Product Info

• Predicted Species Reactivity: Bovine|Dog|Human|Mouse|Rat
• Product Format: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Clonality: Polyclonal
• Host: Goat
• Application: WB, IHC
• Additional Information: Epitope Info: C Terminus
  Domains: Eukaryotic protein kinase domain; SH3 domain; Serine/Threonine protein kinases, catalytic domain; Src homology 2 domains; Src homology 3 domains; Src homology domain 2; Tyrosine kinase, catalytic domain
  Note: Reported variants represent identical protein: NP_004374.1; NP_001120662.1.
  Reactivity Info: Tested: Human, Mouse, Rat; Expected: Human, Mouse, Rat, Dog, Cow
• Reconstitution and Storage: Aliquot and store at -20C. Minimize freezing and thawing.
• Immunogen: CSK
• Purification: Ammonium Sulfate Precipitated; Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Cow: 92%; Dog: 100%; Guinea Pig: 92%; Horse: 92%; Human: 100%; Mouse: 100%; Pig: 100%; Rat: 92%
• Peptide Sequence: C-EQLEHIKTHELHL
• Concentration: 0.5 mg/ml
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Application Info: Peptide ELISA: Antibody detection limit dilution 1:32000.
  Immunohistochemistry: In paraffin embedded Human Colon shows staining of a cell layer between the mucosal columns and the muscularis mucosae. Recommended concentration, 3-6ug/ml.
  Western Blot: Approx 50kDa band observed in lysates of cell line Jurkat and in Mouse and Rat Spleen lysates (calculated MW of 50.7kDa according to NP_004374.1). Recommended concentration: 1-3ug/ml.
• Reference: Holtrich U, Brauninger A, Strebhardt K, Rubsamen-Waigmann H.
  Two additional protein-tyrosine kinases expressed in human lung: fourth member of the fibroblast growth factor receptor family and an intracellular protein-tyrosine kinase.
  Proc Natl Acad Sci U S A 1991 Dec 1;88(23):10411-5 PMID: 1720539

Target Info

• Gene Symbol: Csk|CSK
• Gene Full Name: c-src tyrosine kinase|C-terminal Src kinase
• Alias Symbols: AW212630;CSK, non-receptor tyrosine kinase;C-Src kinase;c-src tyrosine kinase;p50CSK;protein-tyrosine kinase (CSK);protein-tyrosine kinase CYL;protein-tyrosine kinase MPK-2;tyrosine-protein kinase CSK.
• NCBI Gene Id: 12988|1445|315707
• Protein Name: Tyrosine-protein kinase CSK
• Uniprot ID: P41240
• Protein Accession #: NP_004374.1
• Molecular Weight: 50.7