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Catalog No: OKCD07419
Size:96WELLS
Price: $750.00
SKU
OKCD07419
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Datasheets/ManualsPrintable datasheet for CSF3R ELISA Kit (Human) (OKCD07419)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor Receptor, Granulocyte (GCSFR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Colony Stimulating Factor Receptor, Granulocyte (GCSFR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Colony Stimulating Factor Receptor, Granulocyte (GCSFR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Colony Stimulating Factor Receptor, Granulocyte (GCSFR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Colony Stimulating Factor Receptor, Granulocyte (GCSFR) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor Receptor, Granulocyte (GCSFR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
CSF3R Microplate96 Wells (12 x 8 Well strips)
CSF3R Lyophilized Standard2
100X CSF3R HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids.
Sensitivity< 0.056ng/mL
Gene SymbolCSF3R
Gene Full Namecolony stimulating factor 3 receptor
Alias SymbolsCD114;CD114 antigen;colony stimulating factor 3 receptor (granulocyte);G-CSF receptor;GCSFR;G-CSF-R;granulocyte colony-stimulating factor receptor;SCN7.
NCBI Gene Id1441
Protein NameGranulocyte colony-stimulating factor receptor
Description of TargetReceptor for granulocyte colony-stimulating factor (CSF3), essential for granulocytic maturation. Plays a crucial role in the proliferation, differientation and survival of cells along the neutrophilic lineage. In addition it may function in some adhesion or recognition events at the cell surface.
Uniprot IDQ99062
Protein Accession #NP_000751.1
Nucleotide Accession #NM_000760.3
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