CSF3 ELISA Kit (Pig) (OKEH07245)

Catalog No: OKEH07245

Size:96T

Pig CSF3 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Porcine, Sus scrofa
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology CSF3 ELISA Kit (Pig) (OKEH07245) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CSF3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CSF3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CSF3 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 113% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=2.9% (n = 20)
  Mean Inter-assay CV%: <=10.2% (n = 20)
• ELISA Kit Component:
  • CSF3 Microplate: 96 Wells (12 x 8 Well strips)
  • CSF3 Lyophilized Standard: 2
  • 100X Biotinylated CSF3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.
  Subcellular Location: Secreted
  Unigene: Ssc.16151
  SMR: O02837
  String: 9823.ENSSSCP00000018536
  KEGG: ssc:100737548
  Pfam: PF16647
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 39pg/mL
• Specificity: Natural and recombinant Pig Granulocyte colony-stimulating factor
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CSF3
• Gene Full Name: colony stimulating factor 3
• Alias Symbols: colony stimulating factor 3 (granulocyte);G-CSF;granulocyte colony-stimulating factor.
• NCBI Gene Id: 396839
• Protein Name: O02837
• Description of Target: Granulocyte colony-stimulating factor
• Uniprot ID: https://www.uniprot.org/uniprot/O02837
• Protein Accession #: NP_999007.1
• Nucleotide Accession #: NM_213842.1