Size:48 Wells
$328.00
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SKU
OKAA00003-48W
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CSF3 ELISA Kit (Human) : 48 Wells (OKAA00003-48W)

Catalog#: OKAA00003-48W
Domestic: within 1-2 weeks delivery | International: 1-2 weeks
More Information
Predicted Species Reactivity Human
Application ELISA
Kit Detection Method Colorimetric, OD450 nm
Kit Reproducibility The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component
Components
2 vials human G-CSF Standard lyophilized, 2000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human G-CSF monoclonal antibody
2 vials Streptavidin-HRP solution
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
Additional Information Sample Type: cell culture supernates, serum, and plasma
:: Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human G-CSF (8x12)
2. 2 vials human G-CSF Standard lyophilized, 2000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human G-CSF monoclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Sample Type cell culture, supernates, serum, and plasma
Sensitivity 15 pg/ml
Predicted Homology Based on Immunogen Sequence Human
Datasheets/Manuals Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity This assay recognizes both natural and recombinant human APO-1/FAS. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: G-CSF, GM-CSF, IL-1a, IL-1b, IL-2
Recombinant mouse: GM-CSF, TNF-a, MIP-1a, GM-CSF
Other proteins: b FGF acidic, b FGF basic, h PDGF, p PDGF, h TGF-b1
Assay Info Assay Method: Quantitative Sandwich ELISA
Gene Symbol CSF3
Official Gene Full Name colony stimulating factor 3
Alias Symbols C17orf33, CSF3OS, GCSF, G-CSF, Granulocyte colony-stimulating factor, MGC45931, Pluripoietin
NCBI Gene Id 1440
Protein Name Granulocyte colony-stimulating factor
Description of Target The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene.
Swissprot Id P09919
Protein Accession # NP_000750
Nucleotide Accession # NM_000759
Tissue Tool Find tissues and cell lines supported by DNA array analysis to express CSF3.
RNA Seq Find tissues and cell lines supported by RNA-seq analysis to express CSF3.
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