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Catalog No: OKAA00003-48W
Size:48 Wells
Price: $375.00
SKU
OKAA00003-48W
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit ReproducibilityThe coefficient of variation of both intra-assay and inter-assay were less than 10%.
ELISA Kit Component
Components
2 vials human G-CSF Standard lyophilized, 2000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human G-CSF monoclonal antibody
2 vials Streptavidin-HRP solution
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
Additional InformationSample Type: cell culture supernates, serum, and plasma
::Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human G-CSF (8x12)
2. 2 vials human G-CSF Standard lyophilized, 2000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human G-CSF monoclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Sample Typecell culture, supernates, serum, and plasma
Sensitivity15 pg/ml
Predicted Homology Based on Immunogen SequenceHuman
SpecificityThis assay recognizes both natural and recombinant human APO-1/FAS. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: G-CSF, GM-CSF, IL-1a, IL-1b, IL-2
Recombinant mouse: GM-CSF, TNF-a, MIP-1a, GM-CSF
Other proteins: b FGF acidic, b FGF basic, h PDGF, p PDGF, h TGF-b1
Assay InfoAssay Method: Quantitative Sandwich ELISA
Gene SymbolCSF3
Gene Full Namecolony stimulating factor 3
Alias SymbolsGCSF, CSF3OS, C17orf33
NCBI Gene Id1440
Protein NameGranulocyte colony-stimulating factor
Description of TargetThe protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene.
Uniprot IDP09919
Protein Accession #NP_000750
Nucleotide Accession #NM_000759
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