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CSF3 ELISA Kit (Human) : 48 Wells (OKAA00003-48W)

  • Catalog#: OKAA00003-48W
  • 1 week
48 Wells
$328.00
In Stock
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Predicted Species Reactivity:
Human
Application:
ELISA
Additional Information:
Sample Type: cell culture supernates, serum, and plasma
:::
Reagents: REAGENTS
1. Aluminum pouches with a Microwell Plate coated with monoclonal antibody to human G-CSF (8x12)
2. 2 vials human G-CSF Standard lyophilized, 2000 pg/ml upon reconstitution
3. 2 vials concentrated Biotin-Conjugate anti-human G-CSF monoclonal antibody
4. 2 vials Streptavidin-HRP solution,
5. 1 bottle Standard /sample Diluent
6. 1 bottle Biotin-Conjugate antibody Diluent
7. 1 bottle Streptavidin-HRP Diluent
8. 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
9. 1 vial Substrate Solution
10. 1 vial Stop Solution
11. 3 pieces Adhesive Films
12. package insert
Gene Symbol:
CSF3
Official Gene Full Name:
colony stimulating factor 3
Alias Symbols:
C17orf33, CSF3OS, GCSF, G-CSF, Granulocyte colony-stimulating factor, MGC45931, Pluripoietin
NCBI Gene Id:
1440
Protein Name:
Granulocyte colony-stimulating factor
Description of Target:
The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene.
Swissprot Id:
P09919
Protein Accession #:
NP_000750
Nucleotide Accession #:
NM_000759
Sample Type:
cell culture, supernates, serum, and plasma
Sensitivity:
15 pg/ml
Kit Reproducibility:
The coefficient of variation of both intra-assay and inter-assay were less than 10%.
Kit Component:
Components
2 vials human G-CSF Standard lyophilized, 2000 pg/ml upon reconstitution
2 vials concentrated Biotin-Conjugate anti-human G-CSF monoclonal antibody
2 vials Streptavidin-HRP solution
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CSF3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CSF3.
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
This assay recognizes both natural and recombinant human APO-1/FAS. The factors listed below were prepared at 50ng/ml in Standard /sample Diluent and assayed for cross-reactivity and no significant cross-reactivity or interference was observed.
Recombinant human: G-CSF, GM-CSF, IL-1a, IL-1b, IL-2
Recombinant mouse: GM-CSF, TNF-a, MIP-1a, GM-CSF
Other proteins: b FGF acidic, b FGF basic, h PDGF, p PDGF, h TGF-b1
Assay Info:
Assay Method: Quantitative Sandwich ELISA

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