CSF2 Chemi-Luminescent ELISA Kit (Human) (OKCD03538)

Catalog No: OKCD03538

Size:96WELLS

Human CSF2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	98-105%	83-97%	87-99%	84-92%
  EDTA Plasma (n=5)	79-93%	93-101%	92-105%	79-95%
  Heparin Plasma (n=5)	82-95%	93-105%	95-104%	82-99%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) level in the sample or standard.
• ELISA Kit Range: 1.37-1,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	85-92	89
  EDTA Plasma (n=5)	97-104	101
  Heparin Plasma (n=5)	87-94	91

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Colony Stimulating Factor 2, Granulocyte Macrophage (GM-CSF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • CSF2 Microplate: 96 Wells (12 x 8 Well strips)
  • CSF2 Lyophilized Standard: 2
  • 100X CSF2 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 15.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: CSF2
• Gene Full Name: colony stimulating factor 2
• Alias Symbols: colony stimulating factor 2 (granulocyte-macrophage);Colony-stimulating factor;CSF;GMCSF;granulocyte macrophage-colony stimulating factor;granulocyte-macrophage colony-stimulating factor;molgramostim;molgramostin;sargramostim.
• NCBI Gene Id: 1437
• Protein Name: Granulocyte-macrophage colony-stimulating factor
• Description of Target: Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
• Uniprot ID: P04141
• Protein Accession #: NP_000749
• Nucleotide Accession #: NM_000758